DNA Polymerase I (E coli) antibody
Catalogue Number: GTX64108-GTX
GTX64108 Image
GTX64108 Image
GTX64108 Image
Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 µg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP was used.
GTX64108 Image
Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full-size recombinant E. coli DNA polymerase 1. |
| Application: | IP, WB |
Additional Text
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
Western blot. (1:1,000-1:5,000 dilution)
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX64108 Datasheet
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