Legionella pneumophila antibody
Catalogue Number: GTX64135-GTX
GTX64135 Image
GTX64135 Image
GTX64135 Image
Titration of antibody reactivity of anti-Legionella pneumophila antiserum by direct ELISA Plate was coated with 100 µl of 109 cells/ml per well and 100 µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate. "pre" is preimmune serum and "Immunized" is immunized serum
GTX64135 Image
Immunofluorescent staining of Legionella pneumophila in the infected HEK293 cells using anti-Legionella pneumophila antibody. HEK293 cells were infected with Legionella pneumophila strain Philadelphia1 fixed with 4% formaldehyde and reacted with the anti-Legionella pneumophila antibody at 1/10,000 dilution. As the second antibody, goat Rodamine Red X conjugated anti-rabbit IgG antibody was used at 1/10,000 dilution. DNA was stained with Hoechst 33342 (center) and the images were merged with that of differential interference contrast microscope (right).
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Formaldehide treated whole cells of Legionella pneumophila strain Philadelphia 1. Immunized 7 times at two weeks intervals. |
| Application: | ELISA, ICC, IF, IHC, Aggl |
Additional Text
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
Immunofluorescent and Immunochemical staining (1:10,000-1:30,000 dilution), Immunohistochemistry (1:3,000-1:10,000), ELISA (1:10,000-1:30,000 dilution), Agglutination (1:2,000-1:5,000)
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX64135 Datasheet
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