IRF3 antibody
Catalogue Number: GTX64358-GTX
GTX64358 Image
GTX64358 Image
GTX64358 Image
GTX64358 Image
GTX64358 Image
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ChIP analysis of HCT116 cell lysate using GTX64358 IRF3 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX64358 IRF3 antibody. Dilution : 1:1000 Loading : 25µg per lane
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WB analysis of various sample lysates using GTX64358 IRF3 antibody. Dilution : 1:3000 Loading : 25µg per lane
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IHC-P analysis of human esophageal cancer tissue using GTX64358 IRF3 antibody. Dilution : 1:100
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IHC-P analysis of human lung cancer tissue using GTX64358 IRF3 antibody. Dilution : 1:100
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interferon regulatory factor 3 , IIAE7 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1). |
| Application: | IHC-P, ChIP, WB |
Description
Description: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Additional Text
Gene Name
IRF3
Gene ID
3661
Uniprot ID
Q14653
Purification
Affinity Purified
Molecular Weight
47
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. ChIP assay: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX64358 Datasheet
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