Human CD40L / CD154 protein, DDDDK tag (active, multimeric)
Catalogue Number: GTX65635-PRO-GTX
GTX65635-PRO Image
GTX65635-PRO Image
GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100µl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1µg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Lyophilized |
| Type: | Cytokines |
| Alias: | CD40 ligand , CD154 , CD40L , HIGM1 , IGM , IMD3 , T-BAM , TNFSF5 , TRAP , gp39 , hCD40L |
| Host Cell: | CHO Cells |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 ug |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | FA |
Additional Text
Gene Name
CD40LG
Gene ID
959
Protein Tag
FLAG™ Tag
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Application Notes
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED₅₀:: <1ng/ml).
Short Description
Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.
Storage Note
Store at -20C or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
GTX65635-PRO Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations