Human LAG3 protein, human IgG1 Fc tag (active)
Catalogue Number: GTX65640-PRO-GTX
GTX65640-PRO Image
GTX65640-PRO Image
GTX65640-PRO Image
LAG3 human Fc fusion protein (GTX65640-pro) induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x106 cells/ml in complete culture medium, are incubated with LAG3 human Fc fusion protein (GTX65640)(10µg/ml), LPS (positive control, 5µg/ml) or human IgG1 (isotype-matched negative control, 10µg/ml) for 48 hours. DC maturation induced by LAG3 human Fc fusion protein or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
GTX65640-PRO Image
Binding of LAG3 human Fc fusion protein (GTX65640-pro) on dendritic and B cells. Method: Monocyte-derived immature DC (iDC), DC matured for 48h with LPS (mDC) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10µg/ml) or GTX65640 LAG3 human Fc fusion protein (10µg/ml) for 30 min. at 4oC in PBS/1% BSA. The cells are then stained for 30 min. at 4oC with anti-human Fc antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Immune Checkpoint Proteins |
| Alias: | lymphocyte activating 3 , CD223 |
| Host Cell: | CHO Cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | FA |
Additional Text
Gene Name
LAG3
Gene ID
3902
Concentration
0.5 mg/ml
Protein Tag
hIgG1 Fc Tag
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Application Notes
Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells.
Storage Note
Store as concentrated solution. Aliquot and store at -20C or below. Avoid freeze-thaw cycles.
GTX65640-PRO Datasheet
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