Human Progranulins protein (active)
Catalogue Number: GTX65641-PRO-GTX
GTX65641-PRO Image
GTX65641-PRO Image
GTX65641-PRO Image
The effects on phospho-ERK1/2 and ERK1/2 by GTX65641-pro Progranulin protein in neuronal differentiated mouse P19 cells. Undifferentiated mouse P19 cells were induced to differentiated in 1µM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2, reactions were carried out at 37oC over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 cells, which were maintained with serum starvation for 24hrs. Treatment with Progranulin protein (GTX65641-pro) was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively. GAPDH was used as loading control for western blotting.
GTX65641-PRO Image
Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 µg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Lyophilized |
| Type: | Cytokines |
| Alias: | granulin precursor , CLN11 , GEP , GP88 , PCDGF , PEPI , PGRN |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 ug |
| Application: | FA |
Additional Text
Gene ID
2896
Gene Name
GRN
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Application Notes
Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight.
Storage Note
Store at -20C or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
GTX65641-PRO Datasheet
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