CTCF antibody
Catalogue Number: GTX65926-GTX
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
GTX65926 Image
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IP analysis of HeLa cell lysate using GTX65926 CTCF antibody. Antibody amount : 3µg / 200µg lysate Dilution : 1:1000
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WB analysis of 293T cell lysate using GTX65926 CTCF antibody. Dilution : 1:1000 Loading : 25µg per lane
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ChIP analysis of 293T cell lysate using GTX65926 CTCF antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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ICC/IF analysis of U2OS cells using GTX65926 CTCF antibody. Blue : DAPI Dilution : 1:100
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ICC/IF analysis of HeLa cells using GTX65926 CTCF antibody. Blue : DAPI Dilution : 1:100
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IHC-P analysis of human normal cervix tissue using GTX65926 CTCF antibody. Antigen retrieval : 0.01M Citrate Buffer, pH 6.0 Dilution : 1:100
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IHC-P analysis of mouse colon tissue using GTX65926 CTCF antibody. Antigen retrieval : 0.01M Citrate Buffer, pH 6.0 Dilution : 1:100
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IHC-P analysis of human lung cancer tissue using GTX65926 CTCF antibody. Antigen retrieval : 0.01M Citrate Buffer, pH 6.0 Dilution : 1:100
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IHC-P analysis of mouse spleen tissue using GTX65926 CTCF antibody. Antigen retrieval : 0.01M Citrate Buffer, pH 6.0 Dilution : 1:100
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 83 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CCCTC-binding factor , MRD21 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1). |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Additional Text
Gene Name
CTCF
Uniprot ID
P49711
Gene ID
10664
Purification
Affinity Purified
Molecular Weight
83
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:100. ChIP assay: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX65926 Datasheet
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