Hec1 (non-phospho Ser 165) antibody
Catalogue Number: GTX70012-GTX
GTX70012 Image
GTX70012 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (non-phospho Ser 165) antibody (GTX70012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 74 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide (GTX70012-PEP) corresponding to the amino acids around Ser 165 conjugated to KLH |
| Application: | ELISA, ICC, IF, WB |
Description
Description: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Additional Text
Gene ID
10403
Gene Name
NDC80
Uniprot ID
O14777
Concentration
0.91 mg/ml
Purification
Affinity Purified
Molecular Weight
74
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Modification
Non-phosphorylated, Ser165
Application Notes
Recommended Starting Dilutions:For WB: Use at a dilution of 1:600For ICC/IF: Use at a dilution of 1:300Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70012 Datasheet
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