Carbonic Anhydrase IX antibody [GT12]
Catalogue Number: GTX70020-GTX
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Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
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Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
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Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200µM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
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Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200µM CoCl2 (pink) for 48 hr.
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Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
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Sample (30 µg HeLa whole cell lysate) A: 24 hr Untreated B: 24 hr treatment with 100µM CoCl2 C: 24 hr treatment with 200µM CoCl2 D: 48 hr Untreated E: 48 hr treatment with 100µM CoCl2 F: 48 hr treatment with 200µM CoCl2 Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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The data was published in the journal PLoS One in 2015. PMID: 25738958
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The data was published in the journal Oncol Lett in 2015. PMID: 26622817
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The data was published in the journal Oncol Lett in 2015. PMID: 26622817
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The data was published in the journal PLoS One in 2014. PMID: 25420025
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Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | carbonic anhydrase 9 , CAIX , MN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | GT12 |
| Isotype: | IgG2b |
| Immunogen: | |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC |
Description
Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Additional Text
Gene ID
768
Gene Name
CA9
Molecular Weight
50
Uniprot ID
Q16790
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
Carbonic Anhydrase IX antibody (CA9 antibody) detects carbonic anhydrase 9, a ~50 kDa transmembrane glycoprotein. CA9 expression is enhanced by HIF1-alpha signaling in various biological processes, including cell proliferation and transformation. Found in many tissues, CA9 overexpression promotes tumor growth in various cancers.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: Use at a dilution of 1:1000-1:5000.. IHC-P: Use at a dilution of 1:250-1:5000.. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX70020 Datasheet
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