Mre11 antibody [12D7]
Catalogue Number: GTX70212-GTX
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Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human Mre11-transfected 293T cells 7.5% SDS-PAGE Mre11 antibody [12D7] (GTX70212) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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The data was published in the journal Nucleic Acids Res in 2016. PMID: 26446986
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The data was published in the journal Nucleic Acids Res in 2016. PMID: 26446986
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The data was published in the journal Open Biol in 2015.PMID: 25924630
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The data was published in the journal J Virol in 2017.PMID: 28855246
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The data was published in the journal Viruses in 2015.PMID: 26057167
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The data was published in the journal PLoS Pathog in 2010.PMID: 20949077
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The data was published in the journal BMC Biol in 2015. PMID: 25857663
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The data was published in the journal Open Biol in 2015.PMID: 25924630
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The data was published in the journal PLoS One in 2015.PMID: 25826455
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The data was published in the journal Nat Commun in 2017.PMID: 29042561
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The data was published in the journal BMC Cancer in 2014.PMID: 24928521
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The data was published in the journal Nat Commun in 2017.PMID: 29042561
GTX70212 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 81 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 12D7 |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 182-582 of Mre11 expressed in E. coli. |
| Application: | ELISA, ICC, IF, IP, WB, FA, PLA |
Description
Description: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
4361
Uniprot ID
P49959
Concentration
0.45 mg/ml
Purification
Protein G purified
Gene Name
MRE11
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
81
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
MRE11 antibody recognizes MRE11 protein, an enzyme with a predicted molecular weight of ~81 kDa. MRE11 forms the core of the MRN (MRE11-RAD50-NBS1) complex, one of the first components of the DNA damage response (DDR) to DNA double-strand breaks where it recruits other signaling factors required for repair. MRE11 has both exonuclease and endonuclease activities, and is responsible for the DNA binding of the complex.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70212 Datasheet
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