p53 antibody [Pab1801]
Catalogue Number: GTX70216-GTX
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p53 antibody [Pab1801] detects p53 protein at nucleus in NCl-H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded NCl-H1299 xenograft. p53 antibody [Pab1801] (GTX70216) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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p53 antibody [Pab1801] detects p53 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p53 stained by p53 antibody [Pab1801] (GTX70216) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
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p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr A. 5 µg preimmune mouse IgG B. 5 µg of p53 antibody (GTX70216) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
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Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (GTX70216).?(A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by GTX70216 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX225857-01) was used in Western blot.
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The image shows a Western blot for GTX70216 , testing the threshold detection of the p53 antibody. The lanes are as follows:.Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane
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Sample (30 µg of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 µM cisplatin treatment for 24 hr 10% SDS PAGE GTX70216 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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The data was published in the journal Int J Mol Sci in 2019. PMID: 30678307
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab1801] (GTX70216) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 44 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | Pab1801 |
| Isotype: | IgG1 |
| Immunogen: | Gel-purified fusion protein containing the coding region of human p53 expressed in E. coli. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, ChIP, WB, RIA, IHC-Fr |
Description
Description: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Additional Text
Gene Name
TP53
Gene ID
7157
Uniprot ID
P04637
Purification
Protein G purified
Concentration
1.47 mg/ml
Molecular Weight
44
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70216 Datasheet
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