RbAp48 antibody [11G10]
Catalogue Number: GTX70232-GTX
GTX70232 Image
GTX70232 Image
GTX70232 Image
GTX70232 Image
GTX70232 Image
GTX70232 Image
HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX70232 Image
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX70232 Image
RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 µg preimmune mouse IgG B. 5 µg of RbAp48 antibody (GTX70232) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
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Immunofluorescence analysis of HeLa, using RbAp48(GTX70232) antibody at 1:100 dilution.
GTX70232 Image
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 48 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | RB binding protein 4, chromatin remodeling factor , NURF55 , RBAP48 , lin-53 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 11G10 |
| Isotype: | IgG2b |
| Immunogen: | The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC, FA, Blk |
Description
Description: This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Additional Text
Gene Name
RBBP4
Uniprot ID
Q09028
Gene ID
5928
Purification
Protein G purified
Molecular Weight
48
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70232 Datasheet
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