RbAp48 + RbAp46 antibody [15G12]
Catalogue Number: GTX70236-GTX
GTX70236 Image
GTX70236 Image
GTX70236 Image
GTX70236 Image
GTX70236 Image
GTX70236 Image
RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX70236 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX70236 Image
RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Scale bar= 10µm.
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Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.
GTX70236 Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 46 and 48 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 15G12 |
| Isotype: | IgG2a |
| Immunogen: | The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli. |
| Application: | ICC, IF, IHC-P, IP, WB, EMSA |
Description
Description: RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
46 and 48
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 0.2 - 1 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70236 Datasheet
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