Hec1 antibody [9G3.23]
Catalogue Number: GTX70268-GTX
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HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).
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Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500. Blue: Hoechst 33342 staining.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX70268 Image
Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX70268 Image
Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
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The data was published in the journal Nat Commun in 2020. PMID: 32001712
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The data was published in the journal PLoS One in 2014. PMID: 25329824
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The data was published in the journal Nat Commun in 2020. PMID: 32001712
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The data was published in the journal Nat Commun in 2015.PMID: 26620470
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The data was published in the journal Biol Open in 2015.PMID: 26685311
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The data was published in the journal PLoS One in 2010.PMID: 21187932
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The data was published in the journal Oncotarget in 2017.PMID: 29100415
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The data was published in the journal Biol Open in 2015.PMID: 26685311
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The data was published in the journal Oncotarget in 2016.PMID: 27764815
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 74 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 9G3.23 |
| Isotype: | IgG2a |
| Immunogen: | Human HEC1 protein consisting of amino acids 56-642. |
| Application: | FC, ICC, IF, IP, WB, IHC-Fr, PLA |
Description
Description: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Additional Text
Gene ID
10403
Gene Name
NDC80
Uniprot ID
O14777
Molecular Weight
74
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70268 Datasheet
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