MDM2 antibody [SMP14]
Catalogue Number: GTX70278-GTX
GTX70278 Image
GTX70278 Image
GTX70278 Image
IHC-P analysis of human breast carcinoma tissue using GTX70278 MDM2 antibody [SMP14].
GTX70278 Image
Untreated (-) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX70278) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 55 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MDM2 proto-oncogene , ACTFS , HDMX , hdm2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | SMP14 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2 |
| Application: | IHC-P, IP, WB, IHC-Fr, IHC-R |
Description
Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Additional Text
Gene Name
MDM2
Gene ID
4193
Uniprot ID
Q00987
Purification
Protein G purified
Molecular Weight
55
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IHC-P: 1/200-1/500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70278 Datasheet
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