DDDDK tag antibody
Catalogue Number: GTX73143-GTX
GTX73143 Image
GTX73143 Image
GTX73143 Image
GTX73143 Image
GTX73143 Image
ICC/IF analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 1.6 µg/mL(red) with DAPI(blue).
GTX73143 Image
ICC/IF analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 0.8 µg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by acetone.
GTX73143 Image
WB analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody. Lane 1 : 0.4 µg/mL antibody Lane 2 : Secondary antibody only
GTX73143 Image
WB analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody. Lane 1 : 0.4 µg/mL antibody Lane 2 : Secondary antibody only
| Manufacturer: | GeneTex |
| Preservative: | 15 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | FLAG; peptide sequence DYKDDDDK |
| Application: | ICC, IF, IP, WB, DB |
Description
Description: Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Additional Text
Purification
Affinity Purified
Concentration
~0.8 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1-2.5 µg/ml. ICC/IF: 5-10 µg/ml. IP: 4-8 µg. Dot: 1-2.5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX73143 Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations