Pronase reagent (Ready-to-use)

Catalogue Number: GTX73181-GTX

Manufacturer:GeneTex
Physical state:Liquid
Type:Proteases
Shipping Condition:Blue Ice
Unit(s): 100 ml
Application: IHC

Description

Description: Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.

Short Description

Pronase Reagent