IL1 beta antibody
Catalogue Number: GTX74034-GTX
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
GTX74034 Image
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An ELISA plate is coated with 50 µL of IL1 beta recombinant protein at concentration of 5 µg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 µg/mL.
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IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis. Sample: Frozen-sectioned mouse hippocampus. Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX74034 Image
IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250. Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the 2019 in Commun Biol. PMID: 31602405
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The data was published in the 2022 in Sci Rep. PMID: 35256715
GTX74034 Image
The data was published in the 2022 in Sci Rep. PMID: 35256715
GTX74034 Image
The data was published in the 2022 in Sci Rep. PMID: 35256715
GTX74034 Image
The data was published in the journal Nat Commun in 2015.PMID: 26104484
GTX74034 Image
Untreated (-) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX74034 Image
Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX74034 Image
Untreated (-) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 31 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interleukin 1 beta , IL-1beta , Il-1b |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant murine IL1 beta |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Additional Text
Uniprot ID
P10749
Gene Name
IL1B
Gene ID
16176
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
31
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. ELISA: 0.5-2µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Interleukin-1 beta (IL-1 beta) antibody detects IL-1 beta protein, which is a proinflammatory cytokine produced by active macrophages. In recent reports, IL-1 beta activation via caspase-1 can be induced by infectious pathogens in a process known as "pyroptosis", a form of programmed cell death that initiates an inflammatory antimicrobial response. IL-1 beta is also a key factor in cytokine storms associated with both infectious and non-infectious diseases.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX74034 Datasheet
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