GADD34 antibody, C-term
Catalogue Number: GTX74479-GTX
GTX74479 Image
GTX74479 Image
GTX74479 Image
GTX74479 Image
GTX74479 Image
GTX74479 Image
WB analysis of HepG2 (A) and negative control KLY (B) lysate using GTX74479 GADD34 antibody, C-term. Dilution : 0.3µg/ml Loading : 35µg protein in RIPA buffer
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IHC-P analysis of human liver using GTX74479 GADD34 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2µg/ml
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FACS analysis of PFA fixed HepG2 cells using GTX74479 GADD34 antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
GTX74479 Image
ICC/IF analysis of PFA fixed HeLa cells using GTX74479 GADD34 antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX74479 Image
ICC/IF analysis of PFA fixed HepG2 cells using GTX74479 GADD34 antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 73 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | protein phosphatase 1 regulatory subunit 15A , GADD34 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
Additional Text
Concentration
0.5 mg/ml
Gene Name
PPP1R15A
Uniprot ID
O75807
Gene ID
23645
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
73
Application Notes
WB: 0.3-1µg/ml. ICC/IF: 10µg/ml. IHC-P: 2-4µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX74479 Datasheet
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