Protein A antibody (HRP)
Catalogue Number: GTX77595-GTX
GTX77595 Image
GTX77595 Image
GTX77595 Image
An ELISA plate is coated with 50 µL of a Protein A recombinant protein at 10 µg/mL. The coated protein is detected with HRP-conjugated anti-Protein A antibody (GTX77595) at concentration ranged from 0.49 to 500 ng/mL.
GTX77595 Image
Protein A antibody detects Protein A protein by Western blot analysis. Recombinant Protein (0.1ng and 0.1 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Protein A antibody (GTX77595) at a dilution of 1:20000.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 ml |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freund's adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP). |
| Application: | ELISA, WB |
Additional Text
Concentration
0.43 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
For ELISA: Use at a dilution of 1:5,000-1:20,000. For WB: Use at a dilution of 1:1,000-1:20,000. Optimal dilutions/concentrations should be determined by the researcher.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX77595 Datasheet
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