TMB Substrate
Catalogue Number: GTX78422-GTX
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Liquid |
| Type: | Enzyme Substrates |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ml |
| Application: | ELISA |
Description
Description: 3-3',5,5'-tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems. It is recommended for ELISA procedures.
Additional Text
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
Following reaction with peroxidase, a blue reaction product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1 M or 2 M HCl, or 0.5 M H2SO4, equal to the volume of the substrate reaction in the well. The resulting yellow end product, which is stable for at least one hour, can then be read at 450 nm. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
Short Description
3,3',5,5'-Tetramethylbenzidine (TMB) is a chromogenic substrate suitable for use in ELISA procedures, which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction maybe stopped with acid, resulting in a yellow solution that is read at 450 nm. This product is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with pe
GTX78422 Datasheet
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