Cyclophilin 40 antibody
Catalogue Number: GTX79245-GTX
GTX79245 Image
GTX79245 Image
GTX79245 Image
GTX79245 Image
GTX79245 Image
GTX79245 Image
IHC-P analysis of human hepatocarcinoma tissue using GTX79245 Cyclophilin 40 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GTX79245 Image
IHC-P analysis of human skeletal muscle tissue using GTX79245 Cyclophilin 40 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
GTX79245 Image
WB analysis of rat spleen extracts using GTX79245 Cyclophilin 40 antibody.
GTX79245 Image
ICC/IF analysis of A431 cells using GTX79245 Cyclophilin 40 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
GTX79245 Image
ICC/IF analysis of HepG2 cells using GTX79245 Cyclophilin 40 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 41 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | peptidylprolyl isomerase D (cyclophilin D) , 4930564J03Rik , CYP-40 , Ppidl |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC, EMSA |
Description
Description: Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.
Additional Text
Gene Name
PPID
Gene ID
67738
Purification
Purified
Uniprot ID
Q9CR16
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
41
Concentration
1 mg/ml
Application Notes
WB: 1:1,000. ICC/IF: 1:200. IHC-Fr: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX79245 Datasheet
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