Dynein antibody [74.1]
Catalogue Number: GTX80684-GTX
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GTX80684 Image
GTX80684 Image
GTX80684 Image
GTX80684 Image
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FACS analysis of C6 cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of Hela cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
GTX80684 Image
FACS analysis of U87-MG cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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The data was published in the journal Front Oncol in 2019. PMID: 31024851
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ICC/IF analysis of C6 cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
GTX80684 Image
ICC/IF analysis of U-87 MG cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | dynein light chain LC8-type 1 , dynein light chain LC8-type 2 , CDLC2 , DNCL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 74.1 |
| Isotype: | IgG |
| Immunogen: | Purified bovine brain cytoplasmic dynein. |
| Application: | FC, ICC, IF, IP, WB, IHC-WM |
Description
Description: The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Gene ID
404151; 540369
Gene Name
DYNLL1, DYNLL2
UniProt ID
P61285, Q3MHR3
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1 µg/ml. ICC/IF: 2-4 µg/ml. FCM: 1-2 µg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX80684 Datasheet
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