KAP1 antibody [20C1]
Catalogue Number: GTX80695-GTX
GTX80695 Image
GTX80695 Image
GTX80695 Image
GTX80695 Image
GTX80695 Image
WB analysis of nuclear enriched extracts (30 µg lysate) of HEK 293 treated with Hydrogen peroxide (100um Hydrogen peroxide for 10 min) (Lane 1), HEK 293 (Lane 2), A549 (Lane 3), HEL 92.1.7 (Lane 4), PC-3 (Lane 5), and Caco-2 (Lane 6) using GTX80695 KAP1 antibody [20C1]. Dilution : 1 µg/ml
GTX80695 Image
WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].
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ICC/IF analysis of HeLa cells using GTX80695 KAP1 antibody [20C1]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature
GTX80695 Image
ICC/IF analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 89 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 20C1 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein. |
| Application: | ICC, IF, IP, ChIP, WB, EMSA |
Description
Description: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
TRIM28
Purification
Protein G purified
Gene ID
10155
Molecular Weight
89
Uniprot ID
Q13263
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1 - 3 µg/ml. ICC/IF: 2 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX80695 Datasheet
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