PTEN antibody [1B8]
Catalogue Number: GTX83304-GTX
GTX83304 Image
GTX83304 Image
GTX83304 Image
GTX83304 Image
FACS analysis of HeLa cells using GTX83304 PTEN antibody [1B8]. Right : PTEN Left : negative control
GTX83304 Image
ICC/IF analysis of HeLa (left) and HepG2 (right) cells using GTX83304 PTEN antibody [1B8]. Green : PTEN Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
GTX83304 Image
WB analysis of HeLa (1) and NIH3T3 (2) cell lysate using GTX83304 PTEN antibody [1B8].
| Manufacturer: | GeneTex |
| Preservative: | 0.03% Sodium azide |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 1B8 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of PTEN expressed in E. Coli. |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, PLA |
Description
Description: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
5728
Gene Name
PTEN
Uniprot ID
P60484
Molecular Weight
47
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX83304 Datasheet
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