OneStep Polymer HRP anti-rabbit (Ready-to-Use)
Catalogue Number: GTX83399-GTX
GTX83399 Image
GTX83399 Image
GTX83399 Image
PTBP1 antibody detects PTBP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PTBP1 stained by PTBP1 antibody (GTX101481) diluted at 1:500. The signal was developed by OneStep Polymer HRP anti-rabbit (Ready-to-Use) (left), VECTASTAINR ABC-HRP Kits (middle), and conventional secondary antibody (right). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX83399 Image
Triple Positive Breast Cancer sections were stained with rabbit anti-CD3 antibody and then detected by GTX83399 OneStep Polymer HRP anti-rabbit (Ready-to-Use).
| Manufacturer: | GeneTex |
| Preservative: | 0.1% ProClin 150|0.1% ProClin 150 |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Alias: | anti-rabbit HRP polymer , IHC Polymer , antirabbit HRP polymer , anti rabbit HRP polymer |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 tests |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified Rabbit IgG, whole molecule |
| Application: | IHC-P, WB, IHC |
Description
Description: Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.GeneTex's One-Step anti-rabbit IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This reagent has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.Features and Benefits- Lower background: Amplification without biotin avoids background from endogenous biotin- Higher sensitivity than standard IHC detection- Faster: Staining time reduced to 15 minutes! - Compatible with either manual or automated systems- Better results on difficult-to-work antibodies and nuclear staining- Save cost due to further dilution of primary antibody
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
Fully optimized for IHC and can be used with an autostainer or manual immunohistochemistry staining methods.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C.
GTX83399 Datasheet
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