Alpha fetoprotein / AFP antibody [2A9]
Catalogue Number: GTX84954-GTX
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ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
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WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 10 ug per lane Dilution : 1:200
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WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 5 ug per lane
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WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 35 ug per lane
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FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Red : Primary antibody Blue : Negative control antibody
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FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
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IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 69 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | alpha fetoprotein , AFPD , FETA , HPAFP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 2A9 |
| Isotype: | IgG2b |
| Immunogen: | Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Additional Text
Gene Name
AFP
Gene ID
174
Uniprot ID
P02771
Purification
Affinity Purified
Molecular Weight
69
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500. ICC/IF: 1:100. IHC-P: 1:150. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX84954 Datasheet
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