GAPDH antibody

Catalogue Number: GTX85118-GTX

Manufacturer:GeneTex
Preservative:0.02% Sodium azide|0.02% Sodium azide
Molecular Weight:36
Physical state:Liquid
Type:Polyclonal Primary Antibody - Unconjugated
Alias:glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Chicken
Clone:
Isotype: IgY
Immunogen: GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human GAPDH.The immunogen is located within amino acids 60 - 110 of GAPDH.
Application: ELISA, WB

Description

Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Additional Text

Gene Name

GAPDH

Gene ID

2597

Purification

Affinity Purified

Uniprot ID

P04406

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Molecular Weight

36

Concentration

1 mg/ml

Application Notes

WB: 1 - 2 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.