GAD67 antibody
Catalogue Number: GTX85453-GTX
GTX85453 Image
GTX85453 Image
GTX85453 Image
GTX85453 Image
ICC/IF analysis of Sph21 cells using GTX85453 GAD67 antibody. Green : Primary antibody Red : F-actin Blue : DAPI Fixation : 4% paraformaldehyde
GTX85453 Image
ICC/IF analysis of cultured cortical neurons using GTX85453 GAD67 antibody. Red : Primary antibody Green : MAP2 antibody
GTX85453 Image
IHC analysis of mouse brain using GTX85453 GAD67 antibody. The green staining is low level GFP fluorescence expressed off the acting promoter in this mouse transgenic strain. Red : Primary antibody Fixation : 4.0% paraformaldehyde
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 67 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glutamate decarboxylase 1 , CPSQ1 , GAD , SCP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the C-terminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
GAD1
Gene ID
2571
Uniprot ID
Q99259
Concentration
100 ug/ml
Purification
Affinity Purified
Molecular Weight
67
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:2000. IHC: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE.
GTX85453 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations