Annexin V-Biotin Apoptosis Detection Kit

Catalogue Number: GTX85587-GTX

Manufacturer:GeneTex
Shelf Life:12 months
Type:Apoptosis Detection Kit
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 assay
Application: ICC, IF

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Short Description

The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc. - Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy - Sample type- Living cells (suspension and adherant) - Species reactivity- Mammalian - Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.Features and Benefits - Simple one step staining procedure in 30 minutes - Fast and convenient - Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc. Kit Contents: Annexin V-Biotin 1X Binding Buffer Propidium Iodide (PI)

Storage Note

Store at +4C. Product has an expected shelf life of 12 months.