Aurora B (phospho Thr232) antibody
Catalogue Number: GTX85607-GTX
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GTX85607 Image
GTX85607 Image
GTX85607 Image
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Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen
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Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody (GTX85607) at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
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IHC-P analysis of human intestine tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody. Antigen retrieval : not performed. Dilution : 10 µg/mL
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IHC-P analysis of human placenta tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody. Antigen retrieval : not performed. Dilution : 10 µg/mL
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WB analysis of Nocodazole-treated Cos-7 whole cell lysate using GTX85607 Aurora B (phospho Thr232) antibody prior incubated with (Lane 2) or without (Lane 1) blocking peptide. Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 39 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | aurora kinase B , AIK2 , AIM-1 , AIM1 , ARK-2 , ARK2 , AurB , IPL1 , PPP1R48 , STK-1 , STK12 , STK5 , aurkb-sv1 , aurkb-sv2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein. |
| Application: | ELISA, ICC, IF, IHC-P, WB, DB, Multiplex |
Description
Description: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]
Additional Text
Gene Name
AURKB
Gene ID
9212
Molecular Weight
39
Uniprot ID
Q96GD4
Concentration
0.87 mg/ml
Purification
Affinity Purified
Modification
Phosphorylated, Thr232
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:250-1:2000. ELISA: 1:10000-1:30000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX85607 Datasheet
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