Streptavidin (AP)

Catalogue Number: GTX85909-GTX

Manufacturer:GeneTex
Preservative:0.05% Sodium azide|0.05% Sodium azide
Physical state:Liquid
Type:Avidin/Streptavidin Biotin Products
Shipping Condition:Blue Ice
Unit(s): 500 ul
Application: ELISA, WB, IHC

Description

Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band;Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Short Description

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Protein content: > 95%; Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.