Calnexin antibody, Internal (near N-term)
Catalogue Number: GTX88369-GTX
GTX88369 Image
GTX88369 Image
GTX88369 Image
GTX88369 Image
GTX88369 Image
GTX88369 Image
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ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX88369 Calnexin antibody, Internal (near N-term). Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 5µg/ml
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ELISA analysis of Calnexin protein using GTX88369 Calnexin antibody, Internal (near N-term). Capture : GTX112886 (2.5µg/ml) Detection : GTX88369 (1.5µg/ml)
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WB analysis of mouse heart lysate using GTX88369 Calnexin antibody, Internal (near N-term). Dilution : 0.3µg/ml Loading : 35µg protein in RIPA buffer
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IHC-P analysis of human testis using GTX88369 Calnexin antibody, Internal (near N-term). Antigen retrieval : citrate buffer pH 6 Dilution : 3.75µg/ml
GTX88369 Image
IHC-P analysis of human uterus using GTX88369 Calnexin antibody, Internal (near N-term). Antigen retrieval : citrate buffer pH 6 Dilution : 3.75µg/ml
GTX88369 Image
IHC-P analysis of human kidney using GTX88369 Calnexin antibody, Internal (near N-term). Antigen retrieval : citrate buffer pH 6 Dilution : 3.8µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 68 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | calnexin , CNX , IP90 , P90 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB |
Description
Description: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Additional Text
Gene ID
821
Gene Name
CANX
Uniprot ID
P27824
Concentration
0.5 mg/ml
Molecular Weight
68
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.3-1µg/ml. ICC/IF: 1-2µg/ml. IHC-P: 3.75µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX88369 Datasheet
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