CaMKII alpha antibody, Internal
Catalogue Number: GTX88413-GTX
GTX88413 Image
GTX88413 Image
GTX88413 Image
GTX88413 Image
GTX88413 Image
GTX88413 Image
GTX88413 Image
ICC/IF analysis of PFA fixed U251 cells using GTX88413 CaMKII alpha antibody, Internal. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX88413 Image
ICC/IF analysis of PFA fixed U2OS cells using GTX88413 CaMKII alpha antibody, Internal. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX88413 Image
WB analysis of human cerebellum (A), mouse (B) and rat (C) brain lysate using GTX88413 CaMKII alpha antibody, Internal. Dilution : 0.3µg/ml Loading : 35µg protein in RIPA buffer
GTX88413 Image
FACS analysis of PFA fixed Kelly cells using GTX88413 CaMKII alpha antibody, Internal. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
GTX88413 Image
IHC-P analysis of human adrenal gland using GTX88413 CaMKII alpha antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75µg/ml
GTX88413 Image
IHC-P analysis of human cortex using GTX88413 CaMKII alpha antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 54 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | calcium/calmodulin dependent protein kinase II alpha , CAMKA , CaMKIINalpha , MRD53 , MRT63 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_741960.1. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]
Additional Text
Gene Name
CAMK2A
Concentration
0.5 mg/ml
Gene ID
815
Molecular Weight
54
Uniprot ID
Q9UQM7
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.03-0.3µg/ml. ICC/IF: 10µg/ml. IHC-P: 3.75µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX88413 Datasheet
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