Fumarate hydratase antibody, Internal
Catalogue Number: GTX89022-GTX
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GTX89022 Image
GTX89022 Image
GTX89022 Image
GTX89022 Image
GTX89022 Image
WB analysis of human kidney lysate using GTX89022 Fumarate hydratase antibody, Internal. Dilution : 0.01µg/ml Loading : 35µg protein in RIPA buffer
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ELISA analysis of Fumarate hydratase protein using GTX89022 Fumarate hydratase antibody, Internal. Capture : GTX110128 (2µg/ml) Detection : GTX89022 (1.5µg/ml)
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WB analysis of HEK293 overexpressing Fumarate hydratase (mock transfection in first lane) using GTX89022 Fumarate hydratase antibody, Internal.
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IHC-P analysis of human heart using GTX89022 Fumarate hydratase antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75µg/ml
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IHC-P analysis of human skeletal muscle using GTX89022 Fumarate hydratase antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 55 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2. |
| Application: | ELISA, IHC-P, sELISA, WB |
Description
Description: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
FH
Uniprot ID
P07954
Concentration
0.5 mg/ml
Gene ID
2271
Molecular Weight
55
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.01-0.03µg/ml. IHC-P: 3.75µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX89022 Datasheet
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