LEF1 antibody, Internal
Catalogue Number: GTX89284-GTX
GTX89284 Image
GTX89284 Image
GTX89284 Image
GTX89284 Image
GTX89284 Image
FACS analysis of PFA-fixed Jurkat cells using GTX89284 LEF1 antibody, Internal. Blue : Primary antibody Black : Unimmunized goat IgG Permeabilization : 0.5% Triton Dilution : 10µg/ml
GTX89284 Image
ICC/IF analysis of PFA-fixed U2OS cells using GTX89284 LEF1 antibody, Internal. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control (-ve) : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX89284 Image
ICC/IF analysis of PFA-fixed Jurkat cells using GTX89284 LEF1 antibody, Internal. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control (-ve) : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX89284 Image
WB analysis of K562 nuclear extracts using GTX89284 LEF1 antibody, Internal pre-incubated with (Lane B) or without (Lane A) immunogen peptide. Dilution : 2µg/ml Loading : 35µg protein in RIPA buffer
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 44 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1. |
| Application: | FC, ICC, IF, WB |
Description
Description: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Additional Text
Gene ID
51176
Gene Name
LEF1
Uniprot ID
Q9UJU2
Concentration
0.5 mg/ml
Molecular Weight
44
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1-3µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX89284 Datasheet
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