AChE antibody, C-term
Catalogue Number: GTX89580-GTX
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GTX89580 Image
GTX89580 Image
GTX89580 Image
GTX89580 Image
GTX89580 Image
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WB analysis of Daudi (A), HeLa (B) and Jurkat (C) cell lysate using GTX89580 AChE antibody, C-term. Dilution : 0.3µg/ml Loading : 35µg protein in RIPA buffer
GTX89580 Image
WB analysis of rat brain lysate using GTX89580 AChE antibody, C-term. Dilution : 0.3µg/ml Loading : 35µg protein in RIPA buffer
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FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 1µg/ml
GTX89580 Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89580 AChE antibody, C-term. Green : Primary antibody Blue : DAPI Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX89580 Image
WB analysis of HepG2 (A), Daudi (B) and HeLa (C) and Jurkat (D) cell lysate (35µg protein in RIPA buffer) using GTX89580 AChE antibody, C-term. Dilution : 1µg/ml (HepG2 and Daudi) and 0.3µg/ml (HeLa and Jurkat)
GTX89580 Image
FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 68 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1; NP_001269378.1. |
| Application: | FC, ICC, IF, WB |
Description
Description: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
43
Gene Name
ACHE
Uniprot ID
P22303
Concentration
0.5 mg/ml
Molecular Weight
68
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.1-0.5µg/ml. ICC/IF: 10µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX89580 Datasheet
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