PD-L1 antibody, C-term
Catalogue Number: GTX89590-GTX
GTX89590 Image
GTX89590 Image
GTX89590 Image
GTX89590 Image
GTX89590 Image
GTX89590 Image
FACS analysis of PFA-fixed Jurkat cells usingGTX89590 PD-L1 antibody, C-term. Blue line : Primary antibody Black line : Isotype control Permeabilization : 0.5% Triton Dilution : 10ug/ml
GTX89590 Image
ICC/IF analysis of PFA-fixed U2OS cells using GTX89590 PD-L1 antibody, C-term. Green : Primary antibody Blue : DAPI Permeabilization : 0.15% Triton Dilution : 10ug/ml
GTX89590 Image
ICC/IF analysis of PFA-fixed A431 cells using GTX89590 PD-L1 antibody, C-term. Green : Primary antibody Blue : DAPI Permeabilization : 0.15% Triton Dilution : 10ug/ml
GTX89590 Image
WB analysis of U2OS cell lysates using GTX89590 PD-L1 antibody, C-term. Loading : 35µg Dilution : 0.1µg/ml
GTX89590 Image
WB analysis of human heart tissue lysates using GTX89590 PD-L1 antibody, C-term. Loading : 35µg Dilution : 0.01µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1. |
| Application: | FC, ICC, IF, WB |
Description
Description: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Additional Text
Gene Name
CD274
Concentration
0.5 mg/ml
Gene ID
29126
Uniprot ID
Q9NZQ7
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 0.01-0.1µg/ml. ICC/IF: 10µg/ml. FCM: 10µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX89590 Datasheet
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