UBE2C antibody, C-term
Catalogue Number: GTX89866-GTX
GTX89866 Image
GTX89866 Image
GTX89866 Image
GTX89866 Image
GTX89866 Image
WB analysis of HEK293 (A) and HeLa (B) cell lysate using GTX89866 UBE2C antibody, C-term. Dilution : 2µg/ml Loading : 35µg protein in RIPA buffer
GTX89866 Image
FACS analysis of PFA fixed HeLa cells using GTX89866 UBE2C antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
GTX89866 Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX89866 UBE2C antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX89866 Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89866 UBE2C antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 20 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ubiquitin conjugating enzyme E2 C , UBCH10 , dJ447F3.2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1. |
| Application: | FC, ICC, IF, IP, WB |
Description
Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]
Additional Text
Gene Name
UBE2C
Molecular Weight
20
Concentration
0.5 mg/ml
Uniprot ID
O00762
Gene ID
11065
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1-3µg/ml. ICC/IF: 10µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX89866 Datasheet
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