FOXP3 antibody, C-term
Catalogue Number: GTX89934-GTX
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GTX89934 Image
GTX89934 Image
GTX89934 Image
GTX89934 Image
GTX89934 Image
WB analysis of human muscle (A), Molt-4 (B) and negative control pancreas (C) lysate using GTX89934 FOXP3 antibody, C-term. Dilution : 1µg/ml Loading : 35µg protein in RIPA buffer
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IHC-P analysis of human tonsil using GTX89934 FOXP3 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 1µg/ml A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.
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FACS analysis of PFA fixed Jurkat cells using GTX89934 FOXP3 antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
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ICC/IF analysis of PFA fixed U2OS cells using GTX89934 FOXP3 antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
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ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX89934 FOXP3 antibody, C-term. A: Primary antibody and FITC conjigated secondary antibody, B: Phase contrast.
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | forkhead box P3 , AIID , DIETER , IPEX , JM2 , PIDX , XPID |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
FOXP3
Gene ID
50943
Uniprot ID
Q9BZS1
Concentration
0.5 mg/ml
Molecular Weight
47
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1-3µg/ml. ICC/IF: 2-4µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX89934 Datasheet
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