Hemagglutinin Tag (HA Tag) (Agarose)

Catalogue Number: H1200-03A-USB

Manufacturer:	United States Biological
Physical state:	Supplied as 1:1 suspension of beads in PBS, pH7.4, 0.05% azide (0.50ml agarose beads). Binding capacity of the matrix is approx ~200ug HA-tagged protein per ml of the matrix.
Type:	Monoclonal Primary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	11C110
Isotype:	IgG
Immunogen:	A 9aa peptide sequence from hemagglutinin influenza virus (aa114-122; YPY DVP DYA).
Application:	IP

Overview
Datasheets

Description

Description: Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag- epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6xHis) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.

Additional Text

Caution

FOR RESEARCH USE ONLY

Specificity

Recognizes Hemagglutinin Tag.

Purification

Affinity Purified

Antibody Clonality

Monoclonal

H1200-03A Datasheet

Price

£677.00

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