FuGENE® HD Transfection Reagent

Catalogue Number: HD-1000-FUG

Manufacturer:	Fugene
Shelf Life:	4 years
Type:	Transfection Reagents and Kit
Shipping Condition:	RT
Storage Condition:	4°C
Unit(s):	1 ml

Overview
Datasheets

Description

Description: FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. FuGENE® HD interacts with nucleic acids the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is simple and easy-to-use, FuGENE® HD allows researchers and scientists to free up valuable lab time.FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports it into animal orinsect cells. Benefits of FuGENE® HD Reagent include:High transfection efficiency in many common cell types and difficult-to-transfect cell lines, including HeLa, NIH 3T3, COS-1,COS-7, and CHO-K1, HepG2, HEK-293, MCF7, and insect lines. For a list of 1000 cell types that have been successfully transfected with FuGENE® HD Reagent, please contact us.Demonstrates virtually no cytotoxicity, allowing you to work with fewer cells, and eliminates the requirement to changemedia after the addition of transfection complexSuitable for transient and stable-transfectionFunctions exceptionally well in the presence or absence of serum; eliminates the need to change mediaBenefits of FuGENE® HD:Avoid time-consuming optimization procedures by relying on high transfection efficiency proven in more than 1000 cell types, including many “hard-to-transfect cells.Minimal to No ToxicityTransfect in serum-free or serum-containing media.Save time and effort by eliminating multiple handling steps. Simply dilute FuGENE® HD, incubate it with plasmid DNA, and pipet the mixture directly onto your cells.Use less reagent per transfection than competitor reagents.Key ApplicationsVirus Protein ProductionGene ExpressionCellular AnalysisCancer ResearchDifficult-to-Transfect CellsStable Cell Production

HD-1000 Manual