Bulk anti-Human CD3 Antibody (UCHT-1)
Catalogue Number: ICH1002-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Mouse |
| Clone: | UCHT-1 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC, IP, WB, IHC-Fr, FA, MC |
Description
Description: Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
Additional Text
Gene Name
CD3E
Gene ID
916
Uniprot ID
P07766
Purification
Protein A/G Purified
Caution
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibody Clonality
Monoclonal
Concentration
≥ 2 mg/ml
Application Notes
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. Each investigator should determine their own optimal working dilution for specific applications.
Storage Note
anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
ICH1002 Datasheet
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