Anti-Mouse IL-4 (11B11) In Vivo Antibody - Low Endotoxin
Catalogue Number: ICH1087-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Rat |
| Clone: | 11B11 |
| Isotype: | IgG1, kappa |
| Immunogen: | Purified Recombinant Mouse IL-4 (>98%) |
| Application: | ELISA, ELISPOT, FC, IP, IHC, FA, NT, MC |
Description
Description: Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.
Additional Text
Gene Name
IL4
Gene ID
16189
Uniprot ID
P07750
Caution
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration
1-5 mg/ml
Antibody Clonality
Monoclonal
Application Notes
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended. Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Storage Note
anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Purification
This monoclonal antibody was purified using Protein G
Endotoxin
<0.5 EU/mg as determined by the LAL method
ICH1087 Datasheet
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