Anti-Mouse PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12)

Catalogue Number: ICH1091UL-ICB

Manufacturer:IchorBio
Type:Monoclonal Primary Antibody - Unconjugated
Alias:Programmed cell death protein 1, Pdcd1, CD279
Shipping Condition:Blue Ice
Unit(s): 100 mg, 25 mg, 5 mg, 50 mg
Host name: Rat
Clone: 29F.1A12
Isotype: IgG2a, kappa
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Application: FC, WB, IHC-Fr, Blk

Description

Description: Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.

Additional Text

Gene Name

PDCD1

Purification

Protein A/G Purified

Gene ID

18566

Uniprot ID

Q02242

Caution

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Antibody Clonality

Monoclonal

Application Notes

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.

Concentration

≥ 2 mg/ml

Storage Note

anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Price
£3,125.00


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