Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Ultra-Low Endotoxin
Catalogue Number: ICH1091UL-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Programmed cell death protein 1, Pdcd1, CD279 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Rat |
| Clone: | 29F.1A12 |
| Isotype: | IgG2a, kappa |
| Immunogen: | PD-1 cDNA followed by PD-1-Ig fusion protein |
| Application: | FC, WB, IHC-Fr, Blk |
Description
Description: Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Additional Text
Gene Name
PDCD1
Gene ID
18566
Uniprot ID
Q02242
Caution
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibody Clonality
Monoclonal
Application Notes
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.
Concentration
≥ 2 mg/ml
Storage Note
anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Purification
This monoclonal antibody was purified using Protein G
ICH1091UL Datasheet
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