Anti-Mouse Fas Ligand In Vivo Antibody - Low Endotoxin (MFL3) [ICH1178]
Catalogue Number: ICH1178-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6, CD178 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Armenian Hamster |
| Clone: | MFL3 |
| Isotype: | IgG |
| Immunogen: | B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells |
| Application: | FC, IF, Blk |
Description
Description: FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells). Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites. FasL also contributes to CD8 proliferation and neutrophil recruitment. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis.
Additional Text
Uniprot ID
P41047
Purification
Protein A/G Purified
Gene ID
14103
Gene Name
Fasl
Antibody Clonality
Monoclonal
Application Notes
The suggested concentration for this Fas Ligand antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 µg per 10^6 cells in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Concentration
≥ 5 mg/ml
Storage Note
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
ICH1178 Datasheet
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