Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Low Endotoxin

Catalogue Number: ICH1185-ICB

Manufacturer:	IchorBio
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	CD27L receptor, Tnfrsf7
Shipping Condition:	Blue Ice
Unit(s):	100 mg, 25 mg, 5 mg, 50 mg
Host name:	Rat
Clone:	RM27-3E5
Isotype:	IgG1, kappa
Immunogen:	Mouse CD27-human IgG1 Fc fusion protein
Application:	FC, IP, FA

Overview
Datasheets

Description

Description: CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naive T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies. RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo. RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(-) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity

Additional Text

Uniprot ID

P41272

Gene Name

CD27

Gene ID

21940

Antibody Clonality

Monoclonal

Application Notes

Each investigator should determine their own optimal working dilution for specific applications.

Concentration

≥ 5 mg/ml

Storage Note

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Purification

This monoclonal antibody was purified using Protein G

Endotoxin

<1.0 EU/mg as determined by the LAL method

ICH1185 Datasheet

Price

£2,265.00

-

Quantity
Unit Size

Find out about delivery