Anti-Mouse CD27 In Vivo Antibody - Low Endotoxin (RM27-3E5) [ICH1185]

Catalogue Number: ICH1185-ICB

Type:Monoclonal Primary Antibody - Unconjugated
Alias:CD27L receptor, Tnfrsf7
Shipping Condition:Blue Ice
Unit(s): 100 mg, 25 mg, 5 mg, 50 mg
Host name: Rat
Clone: RM27-3E5
Isotype: IgG1, kappa
Immunogen: Mouse CD27-human IgG1 Fc fusion protein
Application: FC, IP, FA


Description: CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naive T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies. RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo. RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(-) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity

Additional Text


Protein A/G Purified

Antibody Clonality


Application Notes

Each investigator should determine their own optimal working dilution for specific applications.


≥ 5 mg/ml


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