Anti-Mouse CD49d (PS/2) In Vivo Antibody - Low Endotoxin
Catalogue Number: ICH1200-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | VLA-4α, ITGA4, Integrin α4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Rat |
| Clone: | PS/2 |
| Isotype: | IgG2b, kappa |
| Immunogen: | PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0. |
| Application: | FC, IF, IHC, Depl |
Description
Description: Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.
Additional Text
Antibody Clonality
Monoclonal
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Concentration
≥ 2 mg/ml
Storage Note
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Purification
This monoclonal antibody was purified using Protein G
Endotoxin
<1.0 EU/mg as determined by the LAL method
ICH1200 Datasheet
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