E coli HB101 Strain Competent Cells
Catalogue Number: IECHB101CC-MIN
| Manufacturer: | Innovative Research |
| Type: | Competent Bacteria |
| Shipping Condition: | Dry Ice |
| Unit(s): | 1 ml |
Description
Description: HB101 strain chemically competent cells from Molecular Innovations are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 5 single use 0.2 ml aliquots. Genotype: F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-
HB101 MSDS
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